Contact an Account ManagerDetermination of catecholamines and related compounds in urine (B)
Summary
Type: HPLC
Application #: 101962
Catalog#: 720183.46 - MN HPLC Column, Nucleosil SB, 100A, 5um, 4.6x150mm
Detection: electrochemical: potential + 0.8V, 12.8 nA full scale
Conditions: eluent for column 1: 108mM citric acid, 92mM NaOH, 0.3mM EDTA, pH 3.5, ionic strength 0.1, saturated 90% with TBP,
eluent for column 2: as for column 1, but without TBP, 5% methanol added,
flow rate 1ml/min
Authors: Eriksson, B.-M. et al.
Source: J. Chromatogr. 527 (1990) 11–19
Details
Legend: Chromatogram after column switching of a human urine sample containing 18.3µM
VMA, 12.6µM iso-VMA (int. std.), sample volume 20µl.